Pengaruh Fenantren terhadap Aktivitas Enzim Katabolik Pseudomonas Putida TI (8)
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Penelitian ini bertujuan untuk menguji pengaruh fenantren terhadap aktivitas enzim katabolik pada Pseudomonas putida TI (8). Isolat ditumbuhkan pada media Air Mineral Sintetis (AMS) dengan penambahan 1% fenantren. Enzim katabolik diuji aktivitasnya terhadap fenantren. Uji aktivitas enzim ditentukan berdasarkan penurunan absorbansi NADH pada panjang gelombang 340 nm. Satu unit aktivitas enzim dinyatakan sebagai jumlah enzim yang membutuhkan 1 µmol NADH untuk mengoksidasi substrat. Hasil penelitian menunjukkan bahwa isolat dapat tumbuh menggunakan fenantren hingga 10 hari inkubasi. Aktivitas enzim katabolik tertinggi terhadap fenantren pada inkubasi hari ke 8 sebesar 5,391 U/mL. Penambahan 1% hidrokarbon poliaromatik dapat meningkatkan aktivitas enzim sebesar 10 kali, hal ini menunjukkan bahwa enzim katabolik pada Pseudomonas putida TI (8) merupakan enzim induktif.
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