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Deteksi dan Identifikasi Senyawa Flavonoid Ekstrak Etanol Kulit Batang Tanaman Majapahit (Crescentia cujete) dengan LCMS

Fatimah Fatimah, Rahma Diyan Martha, Asmarani Kusumawati


Tanaman Majapahit (Crescentia cujete) merupakan salah satu tanaman yang banyak tersebar di Indonesia. Namun, tanaman ini kurang mendapatkan perhatian, karena kurangnya informasi mengenai potensi tanaman tersebut. Flavonoid merupakan salah satu kelompok senyawa terbesar yang terdapat pada tanaman yang memiliki beberapa fungsi farmakologis dan medisinal, salah satunya anti kanker. Dalam penelitian ini, dilakukan deteksi dan identifikasi senyawa flavonoid pada ekstrak etanol kulit batang tanaman majapahit menggunakan LCMS, untuk mengetahui potensi tanaman majapahit sebagai salah satu kandidat tanaman potensial antikanker. Berdasarkan hasil deteksi menggunakan LCMS diketahui terdapat sekitar 88 senyawa yang terdeteksi, termasuk didalamnya 12 senyawa flavonoid. Senyawa flavonoid yang ditemukan, yaitu; acetoin, quercetine, kaempferol-3-O-rhamnoside, acacetin7-rutinoside, fortunellin, kaempferol 3-[6”-(3-hydroxy-3-methylglutaryl) glucoside], didymin, diosmin, hesperidin, rutin, narirutin 4’-glucoside, kaempferol 3-[6”-(3-hydroxy-3-methylglutaryl) glucoside]-7-glucoside. Kaempferol-3 -O-rhamnoside merupakan flavonoid dengan komposisi terbesar yakni, sebesar 4,072%. Keseluruhan flavonoid yang teridentifikasi memiliki potensi sebagai antikanker, kecuali acetoin dan fortunellin.


antikanker; Crescentia cujete; ekstrak etanol; flavonoid; LCMS

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Abotaleb, M., Samuel, S. M., Varghese, E., Varghese, S., Kubatka, P., Liskova, A., & Busselberg, D. (2019). Flavonoids in Cancer and Apoptosis. Cancer, 11(1), 28-66, DOI: 10.3390/cancers11010028

Agati, G., Azzarello, E., Pollastri, S. & Tattini, M. (2012). Flavonoids as Antioxidants in Plants: Location and Functional Signifificance. Plant Science, 196(2012), 67-76, DOI: 10.1016/j.plantsci.2012.07.014

Alghazeer, R., Elgahmasi, S., Elnfati, A, H., Elhensheri, M., Al-Griw, M. A., Awayn., N. & El-Nami, M. (2018). Antioxidant Activity and Hepatoprotective Potential of Flavonoids from Arbutus pavarii Against CCl4 Induced Hepatic Damage. Biotechnology Journal International, 21(1), 1-12, DOI: 10.9734/BJI/2018/39528

Araujo, J. R., Goncalvez., P. & Martel, F. (2011). Chemopreventive Effect of Dietary Polyphenols in Colorectal Cancer Cell lines. Nutrition Research, 31(2), 77-87, DOI: 10.1016/j.nutres.2011.01.006

Das, N., Islam, M. E., Jahan, N., Islam, M. S., Khan, A., Islam, M. R. & Parvin, M. S. (2014). Antioxidant Activities of Ethanol Extracts and Fractions of Crescentia cujete Leaves and Stembark and The Involvement of Phenolic Compounds. BMC Complentary and Alternative Medicine, 14(1), 45, DOI: 10.1186/1472-6882-14-45

Devi, K. P., Rajavel, T., Nabavi, S. F., Setzer, W. N., Ahmadi, A., Mansouri, K., & Nabavi, S. M. (2015). Hesperidin: A Promising Anticancer Agent. Industrial Crops and Products, 6(2015), 582-589, DOI: 10.1016/j.indcrop.2015.07.051

Diab, K.. A. E., Shafik, R. E. S. & Yasuda, S. (2015). In Vitro Antioxidant and Antiproliferative Activities of Novel Orange Peel Extract and It’s Fractions on Leukemia HL-60 Cells. Asian Pacific Journal of Cancer Prevention, 16(16), 7053-7060, DOI: 10.7314/apjcp.2015.16.16.7053

Diab, Y., Atalla, K. & Elbanna, K., (2012). Antimicrobial Screening of Some Egyptian Plants and Active Flavones from Lagerstroemia indica Leaves. Drug Discovery Therapeutic, 6(4), 212-217, DOI: 10.5582/ddt.2012.v6.4.212

Diantini, A., Subarnas, A., Lestari, K., Halimah, E., Susilawati, Y., Supriyatna., Julaeha, E., Achmad, T. H., Suradji, E. W., Yamazaki, C., Kobayashi, K., & Abdulah, R. (2011). Kaempferol-3-O-rhamnoside Isolated From The Leaves of Schima wallichii Korth. Inhibits MCF-7 Breast Cancer Cell Proliferation Through Activation of The Caspase Cascade Pathway. Oncology Letters, 3(2012), 1069-1072, DOI: 10.3892/ol.2012.596

Grassi, D., Desideri, G. & Ferri, C. (2010). Flavonoids: Antioxidants Againts Atherosclerosis. Nutrients, 2(8), 889-902, DOI: 10.3390/nu2080889

Greenwell, M. & Rahman, P. K. S. M. (2015). Medicinal Plants: Their Use in Anticancer Treatment. International Journal of Pharmaceutical Sciences and Research, 6(4), 4103-4112, DOI: 10.13040/IJPSR.0975-8232.6(10).4103-12

Imran, M., Salehi, B., Sharifi-Rad, J., Gondal, T. A. Saeed, F., S., Imran, A.., Shahbaz, M., Fokou, P. V. T., Arshad, M. U., Khan, H., Guerreiro, S. G., Matrins, N., & Estevinho, L. M. (2019). Kaempferol: A Key Emphasis to Its Anticancer Potential. Molecules, 24(12), 2277-2283,

DOI: 10.3390/molecules24122277

Kim, W. K., Bang, M. H., Kim, E. S., Kang, N. E., Jung, K. C., Cho, H. J. & Park, J. H. Y. (2005). Quercetin Decreases The Expression of ErbB2 and ErbB3 Proteins in HT-29 Human Colon Cancer Cells. The Journal of Nutritional Biochemistry, 16(3), 155-162, DOI: 10.1016/j.jnutbio.2004.10.010

Kumar, S., & Pandey, A. K. (2013). Chemistry and Biological Activities of flavonoids: An Overview. The Scientific World Journal, 2013(29), 1-16, DOI: 10.1155/2013/162750

Lalani, S., & Poh, C. L. (2020). Flavonoids as Antiviral Agents for Enterovirus A71 (EV-A71). Viruses, 12(2), 184-219, DOI: 10.3390/v12020184

Lee, K-A., Lee, S-H., Lee, Y-J., Baeg, S. M. & Shim, J-H. (2012). Hesperidin Induces Apoptosis by Inhibiting Sp1 and Its Regulatory Protein MSTO-211H Cells. Biomolecules & Therapeutics, 20(3), 273-279, DOI: 10.4062/biomolther.2012.20.3.273

Lewinska, A., Siwak, J., Rzeszutek, I. & Wnuk, M. (2015). Diosmin Induces Genotoxicity and Apoptosis in DU145 Prostate Cancer Cell line. Toxicology in Vitro, 29(3), 417-425, DOI: 10.1016/j.tiv.2014.12.005

Mota, K. S. D. L., Dias, G. E. N., Pinto, M. E. F., Luiz-Fereira, A., Souza-Brito, A. R. M., Hiruma-Lima, C. A., Barbosa-Filho, J. M., & Batista, L. M. B. (2009). Flavonoids with Gastroprotective Activity. Molecules, 14(3), 979-1012, DOI: 10.3390/molecules14030979

Panche, A. N., Diwan, A. D., & Chandra, S. R. (2016). Flavonoids: An Overview. Journal of Nutritional Science, 5(47), 1-15, DOI: 10.1017/jns.2016.41

Perumal, S & Langeswaran, K. (2019). Diosmin Anti-Tumor Efficacy Against Hepatocellular Carcinoma. Biomedical Research, 30(6), 1-10.

Saibaba, S. V., Kumar, M. S., & Shanmuga, P. (2016). Mini Review on LC/MS Techniques. World Journal of Pharmacy and Pharmaceutical Science, 5(4), 2381-2395

Sghaier, M. B., Pagano, A., Mousslim, M., Ammari, Y., Kovacic, H. & Luis, J. (2016). Rutin Inhibits Proliferation, Attenuates Superoxide Production and Decreases Adhesion and Migration of Human Cancerous Cells. Biomedicine & Pharmacotheraphy, 84(2016), 1972-1978, DOI: 10.1016/j.biopha.2016.11.001

Singh, R. P., Agrawal, P., Yim, D., Agarwal, C. & Agarwal, R. (2005). Acacetin Inhibits Cell Growth and Cell Progression, and Induces Apoptosis in Human Prostate Cancer Cells: Structure-Activity Relationship with Linarin and Linarin Acetate. Carcinogenesis, 26(4), 845-854, DOI: 10.1093/carcin/bgi014

Singhal, S. S., Singhal, S., Singhal, P., Singhal, J., Horne, D. & Awasthi, S. (2017). Didymin: An Orally Active Citrus Flavonoid for Targeting Neuroblastoma. Oncotarget, 8(17), 29428-29441, DOI: 10.18632/oncotarget.15204

Smith, B. A & Dollear, F. G. (1947). Oil From Calabash Seed, Crescentia cujete, L. The Journal of The American Oil Chemist Society, 24(2), 52-54, DOI: 10.1007/BF02642127

Spagnuolo, C., Moccia, S. & Russo, G. L. (2017). Anti-inflammatory Effects of Flavonoids in Neurodegenerative Disorders. European Journal of Medicinal Chemistry, 153(30), 105-115, DOI: 10.1016/j.ejmech.2017.09.001

Stankovic, M. S., Niciforovic, N., Topuzovic, M., & Slavica, S. (2011). Total Phenolic Content, Flavonoid Concentrations and Antioxidant Activity of The Whole Plant And Plant Extracts From Teucrium Montanum L. var Montanum, F. Supinum (L.) Reichen B. Biotechnology and Biotechnology Equipment, 25(1), 2222-2227, DOI: 10.5504/BBEQ.2011.0020

Tattini, M., Gravanno, E., Pinelli, P., Mulinacci, N., & Romani, A. (2000). Flavonoids Accumulate in Leaves and Glandular Trichomes of Phillyrea latifolia Exposed to Excess Solar Radiation. New Phytologist, 148(1), 69-77, DOI: 10.1046/j.1469-8137.2000.00743.x

Veeramuthu, D., Raja, W. T. R., Al-Dhabi, N. A., & Savarimuthu, I. (2017). Flavonoids: Anticancer Properties. Flavonoids-From Biosynthesis to Human Health, Edited by Gonçalo Justino. Chapter 13, Croatia: InTech. DOI: 10.1046/10.5772/68095

Weston, L. A., & Mathesius, U. (2013). Flavonoids: Their Structure, Biosynthesis, and Role in the Rhizosphere, Including Allelopathy. Journal of Chemical Ecology, 39(2), 283-297, DOI: 10.1007/s10886-013-0248-5

Woodman, O. L., Meeker, W. F. & Boujaoude, M. (2005). Vasorelaxant and Antioxidant Activity of Flavonols and Flavones: Structure Activity Relationship. Journal Cardiovascular Pharmacology, 46(3), 302-309, DOI: 10.1097/01.fjc.0000175431.62626.07

Xiao, Z & Lu, J. R. (2014). Generation of Acetoin and Its Derivatives in Foods. Journal of Agricultural and Food Chemistry, 62(28), 6487-6497, DOI: 10.1021/jf5013902

Xiong, Y., Qiu, J., Li, C., Qiu, Y., Guo, L., Liu, Y., Wan, J., Li, Y., Wu, G., Wang, L., Zhou, Z., Dong, J., Du, C., Chen, D. & Guo, H. (2018). Fortunellin-Induced Modulation of Phosphatase and Tensin Homolog by MicroRNA-374a Decreases Inflammation and Maintains Intestinal Barrier Function in Colitis. Frontiers in Immunology, 9(83), 1-11, DOI: 10.3389/fimmu.2018.00083

Yang, F., Song, L., Wang, H., Wang, J., Xu, Z. & Xing, N. (2015). Quercetin in Prostate Cancer: Cheotherapeutic and Chemopreventive Effects, Mechanism and Clinical Application Potentian (Review). Oncology Reports, 33(6), 2659-2668, DOI: 10.3892/or.2015.3886

Zhang, H-W., Hu, J-J. H., Fu, R-Q., Liu, X., Zhang, Y-H., Li, J., Liu, L., Li, Y-N., Deng, Q., Luo, Q-S., Ouyang, Q. & Gao, N. (2018). Flavonoids Inhibits Cell Proliferation and Induce Apoptosis and Autophagy Through Downregulation of PI3KƔ Mediated PI3K / AKT /mTOR/p70S6K/ ULK Signaling Pathway in Human Breast Cancer Cells. Nature, 2018(8), 11255-11267, DOI: 10.1038/s41598-018-29308-7

Zhao, C., Zhang, Y., Liu, H., Li, P., Zhang, H. & Cheng, G. (2017). Fortunellin protects against high fructose-induced diabetic heart injury in mice by suppressing inflammation and oxidative stress via AMPK/Nrf-2 pathway regulation. Biochemical and Biophysical Research Communications, 490(2), 552-559, DOI: 10.1016/j.bbrc.2017.06.076

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