Perbandingan Efektivitas Antibakteri Minyak Atsiri Bawang Putih (Allium sativum) Dan Black Garlic Terhadap Bakteri Staphylococcus Aureus Dan Escherichia coli Dengan Metode Kirby-Bauer
DOI:
https://doi.org/10.25273/pharmed.v1i2.2966Keywords:
garlic (Allium sativum), black garlic, essential oil, kirby-bauerAbstract
Tujuan dari penelitian ini adalah untuk mengetahui daya hambat minyak atsiri pada bawang putih (Allium sativum) dan Black garlic terhadap bakteri Staphylococcus aureus dan Escherichia coli dengan metode Kirby-Bauer. Penelitian ini adalah penelitian eksperimental dengan teknik pengambilan sampel secara sengaja. Hasil dari penelitian ini bahwa kadar minyak atsiri bawang putih (Allium sativum) sebanyak 0,26%, sedangkan pada Black garlic sebanyak 0,15% dari proses destilasi. Daya hambat terhadap bakteri Staphylococcus aureus dari bawang putih (Allium sativum) yaitu 15,5 mm, sedangkan dari Black garlic yaitu 13,5 mm dan daya hambat terhadap bakteri Escherichia coli dari bawang putih (Allium sativum) yaitu 29,33 mm, sedangkan dari Black garlic yaitu 13,16 mm. Hasil penelitian ini menunjukkan bahwa daya hambat minyak atsiri bawang putih (Allium sativum) dan minyak atsiri Black garlic pada bakteri Staphylococcus aureus tidak ada perbedaan yang bermakna sedangkan pada bakteri Escherichia coli ada perbedaan yang bermakna.
Downloads
References
Amagase, B.I., et al. (2001). Intake of Garlic and its Bioactive Components. Journal of Nutrition, (Online), 131(3S), 955S-962S.
Dawis, W and Stout. (1971). Disk plate methods of microbiological antibiotic assay. Microbiology, 22(4), 659-665.
Fujisawa H, et al. (2008). Thermostability Of Allicin Determined By Chemical And Biological Assay. Journal of Bioscience, Biotechnology, and Biochemistry, 72(11), 2877-2883.
Kimura, Shunsuke et al. (2016). Black garlic: A critical review of its production, bioactivity, and application. Journal of food and drug analysis, 25, 62-70.
Ketaren, S. (1985). Pengantar Teknologi Minyak Atsiri. Jakarta: Balai Pustaka.
Radji, M. (2013). Buku Ajar Mikrobiologi. Jakarta: EGC.
Wang, Danan et al. (2010). Black Garlic (Allium sativum) Extracts Enhance The Immune System. Medicinal and Aromatic Plant Science and Biotechnology, 4(1), 37.
Downloads
Published
Issue
Section
License
With the receipt of the article by Pharmed Editorial Board and the decision to be published, the copyright regarding the article will be transferred to Pharmed Journal.
Pharmed has the right to multiply and distribute the article and every author is not allowed to publish the same article that was published in this journal.
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Under the following terms:
Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
NonCommercial — You may not use the material for commercial purposes.
ShareAlike — If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.