Jus Punica Granatum Memulihkan Motilitas Spermatozoa Tikus Putih Pada Kondisi Stres Oksidatif

Putu Oky Ari Tania; Emilia Devi D. Rianti; Indra Firismanda Dermawan; Nur Sri Wahyuni

doi:10.25273/florea.v5i2.3449

Jus Punica Granatum Memulihkan Motilitas Spermatozoa Tikus Putih Pada Kondisi Stres Oksidatif

Putu Oky Ari Tania, Emilia Devi D. Rianti, Indra Firismanda Dermawan, Nur Sri Wahyuni

Abstract

Fast food consumption including used of repeated heating-oil, causing various of health problems, especially the formation of free radicals characterized by elevated MDA levels. Pomegranates have many therapeutic functions, especially as antioxidants that scavenges free radical of oxidative stress thus improving the quality of spermatozoa. The aim of this study was to see the effect of pomegranate juice to reduce oxidative stress and improve sperm motility. This study used 24 rats divided into 4 groups, namely KN, KP, JD1 and JD2. The mean MDA content (nmol / ml) in KN, KP, JD1 and JD2 was 23.35; 8,53; 12.15; and 12,83 followed by Kruskal-Wallis test obtained p value 0,021 (p <0,05). The quality of spermatozoa includes the motility of spermatozoa which distinguished 4 criteria: fast moving spermatozoa and straight forward (criteria 1), spermatozoa move slowly / difficult to go straight (criteria 2), street spermatozoa (criteria 3) and immobile spermatozoa (criteria 4). Mean percentage (%) number of spermatozoa criteria 1 on KN, KP, JD1, and JD2 among others 41,6; 18.2; 38; and 62.2. Mean percentage (%) number of spermatozoa criteria 2 on KN, KP, JD1, and JD2 among others 4,4; 21.8; 15.6 and 14.2. Mean of percentage (%) motility of spermatozoa criteria 3 on KN, KP, JD1, and JD2 among others 10.6; 24.8; 11.2 and 15.2, while for criteria 4 is 21.8; 37.2; 40.4; and 13.8. Statistical test for sperm motility using ANOVA one way, on criteria 1, 2, 3 and 4 each got p value 0,000; 0.010; 0,000; and 0.000, which means the administration of pomegranate juice shows the effect on the motility of spermatozoa.

Full Text:

PDF

References

Agarwal A, Virk G, Ong C, and du Plesis SS, 2014.Effect of Oxidative Stres on Male Reproduction.Word J Mens HealthVol 32 (1) : 1-17

Al-Olayan EM, El-Khadragy MF, Metwally DM, and Moneim AEA, 2014. Protective Effect of Pomegranate (Punica granatum Juice on Testes Against Carbon Tetrachloride Intoxication in Rats. BMC Complementary and Alternative Medicine, 14 : 164

Atilgan D, Parlaktas B, Uluocak N, Gencten Y, Erdemir F, Ozyurt H, Erkorkmaz U and Aslan H, 2014. Pomegranate (Punica granatum) Juice Reduces Oxidative Injury and Improves Sperm Concentration in a Rat Mode of Testicuar Torsio-Detorsion.Experimental and Therpeutic Medicine, 8 : 478-482

Aviram M, Rosenblat M, Gaitini D, Nitecki S, Hoffman A, Dornfeld L, Vokova N, Presse D, Attias J, iker H, and Hayek T, 2004. Pomegranate Juice Consumption for 3 Years by Patients Wihh Carotid Artery Stenosis Reduces Common Carotid Intima-Media Thickness, Bood Pressure and LDL oxidation. Clinical Nutrition Vol 23 : 423-433

Bhandari P, 2012. Pomegranate (Punica granatum L).Ancient Seeds for Modern Cure?Review of Potential Therapeutic Applications.International Journal of Nutrition, Pharmacology, Neurological Diseases, 2(3), 171. doi:10.4103/2231-0738.99469

Bierben E, Sahiner UM, Sackesen C, Erzurum S and Kalayci O, 2012.Oxidative Stress and Antioxidant Defense.WAO Journal, 5 : 5 – 19

Dkhil MA, Al-Quraishy S, and Al-Khalifa MS, 2014. The Effect of Babesia divergens Infection on the Spleen of Mongolian Gerbils. BioMed Research International Vol 2014 : 1-7

Elfalleh W, Hannachi H, Tlili N, Yahlia Y, Nasri N, and Ferchichi A, 2012. Total Phenolic Contents and Antioxidant Activities of Pomegranae Peel, Seed, Leaf and Flower.Journal of Medicine Plants Research Vol 6 (xx) : 4724-4730

Fajrin, FA, 2010. Aktivitas Ekstrak Etanol Ketan Hitam Untuk Menurunkan Kadar Kolesterol. Jurnal Farmasi Indonesia 5(2) : 63–69

Jaarin K and Kamisah Y, 2012. Repeatedly Heated Vegetable Oils and Lipid Peroxidation, chapter 10 : 211-228

Jaarin K, Masbah N, and Nordin SH, 2016.Heated Cooking Oil and Its effect on Blood Pressure and Possible Mechanism: a Review. Int J Clin Exp Med. 9(2): 626-636

Kothari S, Thompson A, Agarwal A and du Plessis SS, 2010. Free Radicals : Their Beneficial and Detrimental Effects on Spem Function. Indian Journal of Experimental Biology Vol 48 : 425-435

Leong XF, Ng CY, Jaarin K, and Mustafa MR, 2015.Effects of Repeated Heating of Cooking Oils on Antioxidant Content and Endothelial Function.Austin J Pharmacol Ther Vol 3 (2) : 1068

Li P, Huo L, Su W, Lu R, Deng C, Liu L, He C, 2011. Free Radical-Scavenging Capacity, Antioxidant Activity and Phenolic Content of Pouzolzia zeylanica.Journal of the Serbian Chemical Society, 76(5), 709–717. doi:10.2298/JSC100818063L

Moneim AEA, Dkhil MA, and Al-quraishy S, 2011. Studies on The Effect of Pomegranate (Punica granatum) juice and Peel On Liver and Kidney in Adult Male Rats, Journal of Medicinal Plants Research Vol 5(20) : 5083–5088

Saleh MA, Clark S, Woodard B, and Deolu-sobogun SA, 2010.Antioxidant and Free Radical Scavenging Activities of Essential Oil.Ethnicity & Disease20 : s78-82

Tirzitis G, and Bartosz G, 2010. Determination of Antiradical and Antioxidant Activity : Basic Principles and New Insights, 57(1), 139–142

Turk G, Sonmez M, Aydin M, Yuce A, Gur S, Yuksel M, Aksu EH, and Aksoy H, 2008. Effect of Pomegranate Juice Consumption on Sperm Quality, Spermatogenic Cell Density, Antioxidant Activity and Testosterone Level in Male Rats.Clinical Nutrition, 27 : 289 – 296

Zeitoun M and Al-Damegh MA, 2015.ffect of Nonenzymatic Antioxidants on Sperm Motility and Survival Relative to Free Radicals and Antioxidant Enzymes of Chilled-Stores Ram Semen.Open Journal of Animals Siences Vol 5 : 50-58