SINTESIS DAN UJI AKTIVITAS SENYAWA 2-BROMO-4,5-DIMETOKSIKALKON TERHDAP SEL KANKER T47D SECARA IN VITRO

Retno Aliyatul Fikroh

Abstract


Synthesis of chalcone derivated has been synthhesized, structurally determined, and the in vitro cytotoxic activityof T47D cell was evaluated. A novel 2-bromo-4,5-dimethoxychalcone was synthesized by Claisen-Schmidt condensation of acetophenone with 6-bromoveratraldehyde. The synthesis of chalcone was carried out by reflux condition (65°C) for 24 hours using ethanol as a solvent and NaOH as a catalyst. The results showed that these coumpoun produced 81% in yield. The purity of synthesized product was determined by Thin Layer Chromatography. Structure identification was performed by FT-IR and 1H dan 13C-NMR spectrometer. In vitro activity test indicated that 2-bromo-4,5-dimethoxychalcone was not active for inhibiting the growth of T47D with IC50 value more than 100 µg/mL.


Keywords


chalcone; synthesis; T47D cancer cell

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References


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DOI: http://doi.org/10.25273/pharmed.v1i1.2269

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