Pengaruh Fenantren terhadap Aktivitas Enzim Katabolik Pseudomonas Putida TI (8)

Meilisa Rusdiana Surya Efendi; Sri Sumarsih; Novia Fajarwati

doi:10.25273/cheesa.v3i2.7735.99-105

Pengaruh Fenantren terhadap Aktivitas Enzim Katabolik Pseudomonas Putida TI (8)

Meilisa Rusdiana Surya Efendi, Sri Sumarsih, Novia Fajarwati

Abstract

Penelitian ini bertujuan untuk menguji pengaruh fenantren terhadap aktivitas enzim katabolik pada Pseudomonas putida TI (8). Isolat ditumbuhkan pada media Air Mineral Sintetis (AMS) dengan penambahan 1% fenantren. Enzim katabolik diuji aktivitasnya terhadap fenantren. Uji aktivitas enzim ditentukan berdasarkan penurunan absorbansi NADH pada panjang gelombang 340 nm. Satu unit aktivitas enzim dinyatakan sebagai jumlah enzim yang membutuhkan 1 µmol NADH untuk mengoksidasi substrat. Hasil penelitian menunjukkan bahwa isolat dapat tumbuh menggunakan fenantren hingga 10 hari inkubasi. Aktivitas enzim katabolik tertinggi terhadap fenantren pada inkubasi hari ke 8 sebesar 5,391 U/mL. Penambahan 1% hidrokarbon poliaromatik dapat meningkatkan aktivitas enzim sebesar 10 kali, hal ini menunjukkan bahwa enzim katabolik pada Pseudomonas putida TI (8) merupakan enzim induktif.

Keywords

bakteri hidrokarbonoklastik; enzim katabolik; fenantren; Pseudomonas putida T1 (8)

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