Penentuan Kadar Alkaloid Total pada Ekstrak Bunga Pepaya (Carica papaya L) dengan Metode Microwave Assisted Extraction

Iman Mukhaimin, Anne Nur Latifahnya, Ernawati Puspitasari

Abstract


Bunga pepaya (Carica papaya L) mengandung senyawa bioaktif alkaloid seperti carpaine, pseudocarpaine dan dehydrocarpaine. Alkaloid tersebut dipercaya bermanfaat untuk sebagai obat antidengue, anti­cancer, antimicrobial, antiparasitic, anti-inflammatory, antioxidant, antidiabetic activities. Pada penelitian ini, bunga pepaya diekstrak menggunakan metode ekstraksi padat-cair menggunakan metode Microwave Assisted Extraction (MAE) dan ekstraksi cair-cair. Sebelum proses ekstraksi, bunga pepaya dikeringkan dan dikecilkan hingga memiliki ukuran partikel sebesar 0,125 mm. Bunga pepaya yang telah kering diekstraksi menggunakan metanol 80% (v/v untuk menentukan pengaruh waktu ekstraksi (1-5 menit), daya microwave (40, 120, 200, 280 Watt), dan rasio sampel terhadap pelarut ( 1:10 ; 1:15 ; 1:20 b/v) terhadap kadar alkaloid total. Hasil ekstrak menunjukkan hasil positif adanya alkaloid setelah diuji dengan pereaksi Mayer dengan terbentuknya endapan berwarna putih kekuningan. Adapun kadar  alkaloid total  terbesar adalah 0,02981 mg/g sampel dengan kondisi operasi ekstraksi waktu ekstraksi 3 menit, rasio sampel terhadap pelarut 1:10 (m/v) serta daya microwave 40 Watt.


Keywords


Alkaloid total, Bunga Pepaya, Ekstraksi, Microwave Assisted Extraction

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DOI: http://doi.org/10.25273/cheesa.v1i2.3340

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