Optimization of Ulva sp. Decomposition using H2SO4 with Microwave-Assisted Hydrolysis Method as Feedstock of Bioethanol

Bekti Palupi; Muhammad Rizalluddin; Kiki Septianti; Istiqomah Rahmawati; Boy Arief Fachri; Meta Fitri Rizkiana; Helda Wika Amini

doi:10.25273/cheesa.v6i1.14682.13-25

Optimization of Ulva sp. Decomposition using H2SO4 with Microwave-Assisted Hydrolysis Method as Feedstock of Bioethanol

Bekti Palupi, Muhammad Rizalluddin, Kiki Septianti, Istiqomah Rahmawati, Boy Arief Fachri, Meta Fitri Rizkiana, Helda Wika Amini

Abstract

Bioethanol is a renewable energy used to reduce dependence on fossil fuels, which have negative impacts on the environment. Furthermore, Ulva sp. contains high levels of carbohydrates, making it potentially suitable as a raw material for bioethanol production. Therefore, this study aims to determine the optimal decomposition process using the microwave-assisted hydrolysis method with an acid solvent (H₂SO₄), by examining the effects of acid concentration, hydrolysis time, and microwave power. Optimization was carried out using several parameters such as hydrolysis time, microwave power, and the ratio of raw materials to solvents. The ANOVA test results showed that the hydrolysis variable parameter had a significant effect on the reducing sugar content obtained, evidenced by the R² value of 0.9892. The highest reducing sugar content of 19.71 mg/mL was produced under the operating conditions of 15 min hydrolysis time, 450 W microwave power, and 0.065 g/mL ratio of raw material to solvents.

Keywords

bioethanol; hydrolysis; microwave-assisted hydrolysis; reducing sugar; Ulva sp.

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